Whole Blood Collection Tubes Sterilization Medical Sterilization Products
7 Collect the blood into 15ml EDTA coated Eppendorf tubes containing 8 ul of 05 M EDTA (See below for the coating procedure) that is chilled on ice and gently mix 8 Gently mix the blood and anticoagulant and store on ice 9 Estimate the volume of the blood and add additional amount of 05 M EDTA so the final concentration of EDTA is 5 mMThe following materials are used to collect and store frozen blood Item Description Blood ≥ 650 µL (≥ 13 mL preferred) for samples with nonnucleated RBCs Collection tube (EDTA preferred eg BD Sciences, catalog # Cryovials with lid gaskets eg ThermoFisher Scientific Catalog # Disinfecting spray or wipes
